In situ potentiometry and ellipsometry: a promising tool to study biofouling of potentiometric sensors

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Grzegorz Lisak, Thomas Arnebrant, Andrzej Lewenstam, Johan Bobacka, Tautgirdas Ruzgas
Publisher: ACS Publications
Publication year: 2016
Journal: Analytical Chemistry
Journal acronym: Anal. Chem.
Volume number: 88
Issue number: 6
Start page: 3009
End page: 3014
eISSN: 1520-6882


Abstract

In situ potentiometry and null ellipsometry was combined and used as a tool to follow the kinetics of biofouling of ion-selective electrodes (ISEs). The study was performed using custom-made solid-contact K+-ISEs consisting of a gold surface with immobilized 6-(ferrocenyl)hexanethiol as ion-to-electron transducer that was coated with a potassium-selective plasticized polymer membrane. The electrode potential and the ellipsometric signal (corresponding to the amount of adsorbed protein) were recorded simultaneously during adsorption of bovine serum albumin (BSA) at the surface of the K+-ISEs. This in situ method may become useful in developing sensors with minimized biofouling.

Last updated on 2020-22-01 at 04:16