Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Diosángeles Soto Véliz, Catharina Alam, Thiago Nietzel, Rebecca Wyborski, Adolfo Rivero-Müller, Parvez Alam
Publication year: 2015
Journal: Colloids and Surfaces B: Biointerfaces
Volume number: 133
Start page: 140
End page: 147
eISSN: 1873-4367


Abstract

In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.

Last updated on 2019-21-11 at 04:15