The influence of mineral particles on fibroblast behaviour: A comparative study

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Diosangeles Soto Veliz, Jens C Luoto, Ilari Pulli, Martti Toivakka
Publisher: Elsevier
Publication year: 2018
Journal: Colloids and Surfaces B: Biointerfaces
Journal acronym: Colloids Surf B Biointerfaces
Volume number: 167
Start page: 239
End page: 251
eISSN: 1873-4367


Minerals are versatile tools utilised to modify and control the physical-chemical and functional properties of substrates. Those properties include ones directing cell fate; thus, minerals can potentially provide a direct and inexpensive method to manipulate cell behaviour. This paper shows how different minerals influence human dermal fibroblast behaviour depending on their properties. Different calcium carbonates, calcium sulphates, silica, silicates, and titanium dioxide were characterised using TEM, ATR-FTIR, and zeta potential measurements. Mineral-cell interactions were analysed through MTT assay, LDH assay, calcein AM staining, live cell imaging, immunofluorescence staining, western blot, and extra/intracellular calcium measurements. Results show that the interaction of the fibroblasts with the minerals was governed by a shared period of adaptation, followed by increased proliferation, growth inhibition, or increased toxicity. Properties such as size, ion release and chemical composition had a direct influence on the cells leading to cell agglomeration, morphological changes, and the possible formation of protein-mineral complexes. In addition, zeta potential and FTIR measurements of the minerals showed adsorption of the cell culture media onto the particles. This article provides fundamental insight into the mineral-fibroblast interactions, and makes it possible to arrange the minerals according to the time-dependent cellular response.


behavior, Biocompatibility, fibroblast, minerals, particle

Last updated on 2019-05-12 at 03:10