Purification and Validation of Lipid Transfer Proteins

A3 Book section, Chapters in research books


Internal Authors/Editors


Publication Details

List of Authors: Matti A. Kjellberg, Anders P.E. Backman, Anna Möuts, Peter Mattjus
Editors: Sanjoy K. Bhattacharya
Publication year: 2017
Publisher: Humana Press
Book title: Lipidomics - Methods and Protocols
Title of series: Methods in Molecular Biology
Volume number: 1609
ISBN: 978-1-4939-6995-1


Abstract

Understanding the holistic picture of lipid homeostasis involves not only the analysis of synthesis and breakdown of lipids, but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.


Keywords

Fluorescence, lipid, surface plasmon resonance, vesicle

Last updated on 2019-21-10 at 04:24

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