How to Train a Cell-Cutting-Edge Molecular Tools

A2 Review article, Literature review, Systematic review


Internal Authors/Editors


Publication Details

List of Authors: Czapinski J, Kielbus M, Kalafut J, Kos M, Stepulak A, Rivero-Muller A
Publisher: FRONTIERS MEDIA SA
Publication year: 2017
Journal: Frontiers in Chemistry
Journal acronym: FRONT CHEM
Volume number: 5
Number of pages: 25
ISSN: 2296-2646


Abstract

In biological systems, the formation of molecular complexes is the currency for all cellular processes. Traditionally, functional experimentation was targeted to single molecular players in order to understand its effects in a cell or animal phenotype. In the last few years, we have been experiencing rapid progress in the development of ground-breaking molecular biology tools that affect the metabolic, structural, morphological, and (epi) genetic instructions of cells by chemical, optical (optogenetic) and mechanical inputs. Such precise dissection of cellular processes is not only essential for a better understanding of biological systems, but will also allow us to better diagnose and fix common dysfunctions. Here, we present several of these emerging and innovative techniques by providing the reader with elegant examples on how these tools have been implemented in cells, and, in some cases, organisms, to unravel molecular processes in minute detail. We also discuss their advantages and disadvantages with particular focus on their translation to multicellular organisms for in vivo spatiotemporal regulation. We envision that further developments of these tools will not only help solve the processes of life, but will give rise to novel clinical and industrial applications.


Keywords

cell communication, controlling behavior, gene editing, Gene Expression Regulation, optogenetics, protein interactions, signaling pathways, synthetic biology

Last updated on 2019-13-11 at 03:41

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