FiloQuant reveals increased filopodia density during breast cancer progression

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Jacquemet G, Paatero I, Carisey AF, Padzik A, Orange JS, Hamidi H, Ivaska J
Förläggare: ROCKEFELLER UNIV PRESS
Publiceringsår: 2017
Tidskrift: Journal of Cell Biology
Tidskriftsakronym: J CELL BIOL
Volym: 216
Nummer: 10
Artikelns första sida, sidnummer: 3387
Artikelns sista sida, sidnummer: 3403
Antal sidor: 17
ISSN: 0021-9525


Abstrakt

Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack of compatible methods to quantify filopodia properties. Here, we present FiloQuant, a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed-and live-cell microscopy data. We demonstrate that FiloQuant can extract quantifiable information, including protrusion dynamics, density, and length, from multiple cell types and in a range of microenvironments. In cellular models of breast ductal carcinoma in situ, we reveal a link between filopodia formation at the cell-matrix interface, in collectively invading cells and 3D tumor spheroids, and the in vitro invasive capacity of the carcinoma. Finally, using intravital microscopy, we observe that tumor spheroids display filopodia in vivo, supporting a potential role for these protrusions during tumorigenesis.

Senast uppdaterad 2019-12-11 vid 03:37