Antibacterial effects of wood structural components and extractives from Pinus sylvestris and Picea abies on methicillin-resistant Staphylococcus aureus and Escherichia coli O157:H7

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Tiina Vainio-Kaila, Xue Zhang, Tuomas Hänninen, Aino Kyyhkynen, Leena-Sisko Johansson, Stefan Willför, Monika Österberg, Anja Siitonen, Lauri Rautkari
Publication year: 2017
Journal: BioResources
Volume number: 12
Issue number: 4
Start page: 7601
End page: 7614
eISSN: 1930-2126


Abstract

Antibacterial properties of wood structural components and extractives were investigated against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli
O157:H7 by placing bacterial inoculum on the model surfaces and
incubating them for 2, 4, and 24 h. After incubation, the amount of
viable bacteria on the surfaces was studied. The film coverage and
thickness were evaluated with atomic-force microscopy (AFM) and X-ray
photoelectron spectroscopy (XPS). The extracts were analyzed with gas
chromatography–mass spectrometry (GC-MS). The results showed that films
fully covered the glass surfaces. The XPS results confirmed the analysis
of GC-MS, which revealed more similarities between the extractives of
pine heartwood and spruce heartwood than between pine heartwood and pine
sapwood. Only the pine heartwood extract showed an antibacterial effect
against E. coli O157:H7. In contrast, MRSA was susceptible to all of the extracts and milled wood lignin (MWL).


Last updated on 2019-21-11 at 04:48