Quantification and Qualitative Effects of Different PEGylations on Poly(butyl cyanoacrylate) Nanoparticles

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Åslund AKO, Sulheim E, Snipstad S, von Haartman E, Baghirov H, Starr N, Kvåle Løvmo M, Lelú S, Scurr D, Davies CdL, Schmid R, Mørch Ý
Förläggare: American Chemical Society
Publiceringsår: 2017
Tidskrift: Molecular Pharmaceutics
Volym: 14
Nummer: 8
Artikelns första sida, sidnummer: 2560
Artikelns sista sida, sidnummer: 2569
ISSN: 1543-8384


Abstrakt

Protein adsorption on nanoparticles (NPs) used in nanomedicine leads to
opsonization and activation of the complement system in blood, which
substantially reduces the blood circulation time of NPs. The most
commonly used method to avoid protein adsorption is to coat the NPs with
polyethylene glycol, so-called PEGylation. Although PEGylation is of
utmost importance for designing the in vivo behavior of the NP,
there is still a considerable lack of methods for characterization and
fundamental understanding related to the PEGylation of NPs. In this work
we have studied four different poly(butyl cyanoacrylate) (PBCA) NPs,
PEGylated with different types of PEG-based nonionic
surfactants—Jeffamine M-2070, Brij L23, Kolliphor HS 15, Pluronic F68—or
combinations thereof. We evaluated the PEGylation, both quantitatively
by nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA),
and time-of-flight secondary ion mass spectrometry (ToF-SIMS) and
qualitatively by studying ζ-potential, protein adsorption, diffusion,
cellular interactions, and blood circulation half-life. We found that
NMR and ToF-SIMS are complementary methods, while TGA is less suitable
to quantitate PEG on polymeric NPs. It was found that longer PEG
increases both blood circulation time and diffusion of NPs in collagen
gels.


Senast uppdaterad 2019-09-12 vid 03:46

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