Gold nanoparticle printed coverslips to facilitate fluorescence-TEM correlative microscopy

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Neeraj Prabhakar, Anni Määttänen, Jouko Peltonen, Pekka Hänninen, Markus Peurla, Jessica M Rosenholm
Publisher: Oxford Academic
Publication year: 2017
Journal: Microscopy


Abstract

Correlative light and electron microscopy (CLEM) allows combining the
advantages of fluorescence microscopy and electron microscopy for cell
imaging. Rare phenomenon expressing cells can be studied by specifically
tagged fluorophores with fluorescence microscopy. Subsequently, cells
can be fixed and ultra-structural details can be studied with
transmission electron microscopy (TEM) at a higher resolution. However,
precise landmarks are necessary to track the same cell throughout the
CLEM process. In this technical report, we present a high contrast
inkjet-printed gold nanoparticle patterns over commercial glass
coverslip to facilitate cell tracking with correlative microscopy. High
contrast and strong reflection from nano gold pattern can be used as a
fixed landmark for cell identification with fluorescence microscopy.
Nano gold printed letters over coverslips are visible in resin blocks,
which can be further used to identify the cell of interest for
performing sectioning of embedded cell blocks for TEM.


Last updated on 2019-05-12 at 05:30