Molecular characterization and homology modeling of spermidine synthase from Synechococcus sp. PCC 7942.

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Pothipongsa A, Jantaro S, Salminen TA, Incharoensakdi A
Publication year: 2017
Journal: World Journal of Microbiology and Biotechnology
Journal acronym: World J Microbiol Biotechnol
Volume number: 33
Issue number: 4
ISSN: 1573-0972


Abstract

Spermidine synthase (Spds) catalyzes the formation of spermidine by transferring the aminopropyl group from decarboxylated S-adenosylmethionine (dcSAM) to putrescine. The Synechococcus spds gene encoding Spds was expressed in Escherichia coli. The purified recombinant enzyme had a molecular mass of 33 kDa and showed optimal activity at pH 7.5, 37 °C. The enzyme had higher affinity for dcSAM (K m, 20 µM) than for putrescine (K m, 111 µM) and was highly specific towards the diamine putrescine with no activity observed towards longer chain diamines. The three-dimensional structural model for Synechococcus Spds revealed that most of the ligand binding residues in Spds from Synechococcus sp. PCC 7942 are identical to those of human and parasite Spds. Based on the model, the highly conserved acidic residues, Asp89, Asp159 and Asp162, are involved in the binding of substrates putrescine and dcSAM and Pro166 seems to confer substrate specificity towards putrescine.

Last updated on 2019-24-10 at 03:14