Detoxification of acid pretreated spruce hydrolysates with ferrous sulfate and hydrogen peroxide improves enzymatic hydrolysis and fermentation

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Venkata Prabhakar Soudham, Tomas Brandberg, Jyri-Pekka Mikkola, Christer Larsson
Publication year: 2014
Journal: Bioresource Technology
Volume number: 166
Start page: 559
End page: 565
eISSN: 1873-2976


The aim of the present work was to investigate whether a detoxification method already in use during waste water treatment could be functional also for ethanol production based on lignocellulosic substrates. Chemical conditioning of spruce hydrolysate with hydrogen peroxide (H2O2) and ferrous sulfate (FeSO4) was shown to be an efficient strategy to remove significant amounts of inhibitory compounds and, simultaneously, to enhance the enzymatic hydrolysis and fermentability of the substrates. Without treatment, the hydrolysates were hardly fermentable with maximum ethanol concentration below 0.4 g/l. In contrast, treatment by 2.5 mM FeSO4 and 150 mM H2O2 yielded a maximum ethanol concentration of 8.3 g/l.

Last updated on 2020-07-06 at 03:16