Improvement in carbohydrate and phlorotannin extraction from Macrocystis pyrifera using carbohydrate active enzyme from marine Alternaria sp. as pretreatment

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: A. Leyton, R. Pezoa-Conte, P. Mäki-Arvela, J-P Mikkola, M. E Lienqueo
Publisher: Springer
Publication year: 2017
Journal: Journal of Applied Phycology
Journal acronym: J. Appl. Phycol.
Volume number: 29
Issue number: 4
Start page: 2039
End page: 2048
eISSN: 1573-5176


The commercial importance of brown seaweed has been increasing over the past decade, especially due to industries interested in the extraction of phycocolloids and, more recently, of polyphenol compounds such as phlorotannins. The objective of this work was to optimize the extraction conditions of carbohydrates and phlorotannins from Macrocystis pyrifera, evaluated enzymatic pretreatment and different parameters of extraction using design of experiment. The optimal conditions upon extraction of the carbohydrates and phlorotannins were determined by means of a pretreatment protocol taking advantage on a carbohydrate active enzyme, followed by an alkaline hydrolysis with 0.5 N NaOH at 100 °C, 180 min, and S/L ratio of 1/20. In order to extract the carbohydrates, the best conditions found for the pretreatment procedure were 37 °C, pH 7.0 for 24 h, and a S/L ratio of 1/10, giving an extraction yield (EY) of 89.67 ± 12.3 wt.%. In turn, for the extraction of phlorotannins, the best conditions identified in terms of the pretreatment were 25 °C, pH 7.0 for 36 h, and a S/L ratio of 1/20, thus giving a yield (EY) of 2.14 ± 0.25 wt.%. Statistical analysis of both processes revealed a maximum EY of 91.24 wt.% for carbohydrates and 3.31 wt.% EY for phlorotannins.


Box-Behnken design, Brown seaweed, experimental design, Extraction, Taguchi design

Last updated on 2020-29-05 at 06:52