A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: E. Rosqvist, E. Niemelä, J. Frisk, H. Öblom, R. Koppolu, H. Abdelkader, D. Soto Véliz, M. Mennillo, A. P. Venu, P. Ihalainen, M. Aubert, N. Sandler, C.-E. Wilén, M. Toivakka, J. E. Eriksson, R. Österbacka, J. Peltonen
Förläggare: Royal Society of Chemistry
Publiceringsår: 2020
Tidskrift: Journal of Materials Chemistry. B
Tidskriftsakronym: J. Mater. Chem. B
Volym: 8
Nummer: 6
Artikelns första sida, sidnummer: 1146
Artikelns sista sida, sidnummer: 1156
eISSN: 2050-7518


A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.

Senast uppdaterad 2020-07-07 vid 04:38