Analysis of Label-Free Single-Molecule Biosensors based on Gate-Biofunctionalized Organic Transistors

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Publication Details

List of Authors: Torricelli F, Macchia E, Manoli K, Di Franco C, Kovacs-Vajna ZM, Palazzo G, Scamarcio G, Torsi L
Publication year: 2019
Publisher: IEEE
Book title: 2019 IEEE 8th International Workshop on Advances in Sensors and Interfaces (IWASI)
Start page: 70
End page: 74
Number of pages: 5
ISBN: 978-1-7281-0558-1
eISBN: 978-1-7281-0557-4


A label-free single-molecule detection platform based on biofunctionalized organic transistors has been recently proposed. In contrast to state-of-art approaches based on nano-transducers, the aforementioned single-molecule transistor (SiMoT) sensing technology is based on millimeter-sized transistors where the gate is bio-functionalized with about a trillion of antibodies. In this work we provide a detailed investigation of SiMoT biosensors by studying and quantifying the device parameters affected by the biorecognition event. The study combines both measurements and theoretical analyses, showing that a single protein detection taking place at nanometer scale affects a millimeter scale area of the biofunctionalized gate electrode. The dimension of the bioprobe domains affected by the binding is calculated as a function of the nominal protein concentration in the analyte solution. These results provide important insight into the SiMoT biosensor technology, which can guide the development of multi-modal SiMoT detection and integrated SiMoT biosensors arrays.


biofunctionalization, biosensor, organic transistor, single molecule

Last updated on 2020-26-05 at 03:38