Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Koe, Tan, Lönnfors, Hur, Low, Zhang, Kanchanawong, Bankaitis, Wang
Publiceringsår: 2018
Tidskrift: eLife
Tidskriftsakronym: Elife
Volym: 7
ISSN: 2050-084X


Abstrakt

A central feature of most stem cells is the ability to self-renew and
undergo differentiation via asymmetric division. However, during
asymmetric division the role of phosphatidylinositol (PI) lipids and
their regulators is not well established. Here, we show that the sole
type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vibor disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.

Senast uppdaterad 2020-10-08 vid 07:07