Chemical and Photonic Interactions in vitro and in vivo between Fluorescent Tracer and Nanoparticle-based Scavenger for enhanced Molecular Imaging

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Gulin-Sarfraz T, Pryazhnikov E, Zhang J, Khiroug L, Rosenholm JM
Publisher: Elsevier
Publication year: 2019
Journal: Materials Today Bio
Volume number: 2
ISSN: 2590-0064
eISSN: 2590-0064


Abstract

We hereby present a concept of scavenging excess imaging agent prior to a
diagnostic imaging session, consequently allowing for enhanced contrast
of signals originating from the tissue area of interest to the signals
originating from systemic imaging agent residues. In our study, a
prospective silica core–shell nanoparticle-based scavenger was designed
and explored for its feasibility to scavenge a specific imaging agent
(tracer) in the bloodstream. The developed tracer–scavenger system was
first investigated under in vitro conditions to ensure proper
binding between tracer and scavenger is taking place, as confirmed by
Förster/fluorescence resonance energy transfer studies. In vivo,
two-photon imaging was utilized to directly study the interaction of
the scavenger particles and the tracer molecules in the vasculature of
mice. To our knowledge, a methodological solution for in vivo differentiation between signals, originating from tissue and blood, has not been presented elsewhere.


Keywords

brain imaging, Contrast agent, Diagnostics, diagnostic tools, diagnostic use, Silica


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Last updated on 2020-25-02 at 06:48