Cellulose nanofibrils prepared by gentle drying methods reveal the limits of helium ion microscopy imaging

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Annika E. Ketola, Miika Leppänen, Tuomas Turpeinen, Petri Papponen, Anders Strand, Anna Sundberg, Kai Arstila, Elias Retulainen
Förläggare: Royal Society of Chemistry
Publiceringsår: 2019
Tidskrift: RSC Advances
Tidskriftsakronym: RSC Adv.
Volym: 9
Artikelns första sida, sidnummer: 15668
Artikelns sista sida, sidnummer: 15677
Antal sidor: 10
ISSN: 2046-2069
eISSN: 2046-2069


Abstrakt

TEMPO-oxidized cellulose nanofibrils (TCNFs) have unique properties, which can be utilised in many application fields from printed electronics to packaging. Visual characterisation of TCNFs has been commonly performed using Scanning Electron Microscopy (SEM). However, a novel imaging technique, Helium Ion Microscopy (HIM), offers benefits over SEM, including higher resolution and the possibility of imaging non-conductive samples uncoated. HIM has not been widely utilized so far, and in this study the capability of HIM for imaging of TCNFs was evaluated. Freeze drying and critical point drying (CPD) techniques were applied to preserve the open fibril structure of the gel-like TCNFs. Both drying methods worked well, but CPD performed better resulting in the specific surface area of 386 m(2) g(-1) when compared to 172 m(2) g(-1) and 42 m(2) g(-1) of freeze dried samples frozen in propane and nitrogen, respectively. HIM imaging of TCNFs was successful but high magnification imaging was challenging because the ion beam tended to degrade the TCNFs. The effect of the imaging parameters on the degradation was studied and an ion dose as low as 0.9 ion per nm(2) was required to prevent the damage. This study points out the differences between the gentle drying methods of TCNFs and demonstrates beam damage during imaging like none previously reported with HIM. The results can be utilized in future studies of cellulose or other biological materials as there is a growing interest for both the HIM technique and bio-based materials.


Nyckelord

microscopy, Nanocellulose

Senast uppdaterad 2020-01-04 vid 08:21