GFAP and Vimentin Deficiency Alters Gene Expression in Astrocytes and Microglia in Wild-Type Mice and Changes the Transcriptional Response of Reactive Glia in Mouse Model for Alzheimer's Disease

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Kamphuis W, Kooijman L, Orre M, Stassen O, Pekny M, Hol EM
Publisher: WILEY-BLACKWELL
Publication year: 2015
Journal: Glia
Journal acronym: GLIA
Volume number: 63
Issue number: 6
Start page: 1036
End page: 1056
Number of pages: 21
ISSN: 0894-1491


Abstract

Reactive astrocytes with an increased expression of intermediate filament (IF) proteins Glial Fibrillary Acidic Protein (GFAP) and Vimentin (VIM) surround amyloid plaques in Alzheimer's disease (AD). The functional consequences of this upregulation are unclear. To identify molecular pathways coupled to IF regulation in reactive astrocytes, and to study the interaction with microglia, we examined WT and APPswe/PS1dE9 (AD) mice lacking either GFAP, or both VIM and GFAP, and determined the transcriptome of cortical astrocytes and microglia from 15- to 18-month-old mice. Genes involved in lysosomal degradation (including several cathepsins) and in inflammatory response (including Cxcl5, Tlr6, Tnf, Il1b) exhibited a higher AD-induced increase when GFAP, or VIM and GFAP, were absent. The expression of Aqp4 and Gja1 displayed the same pattern. The downregulation of neuronal support genes in astrocytes from AD mice was absent in GFAP/VIM null mice. In contrast, the absence of IFs did not affect the transcriptional alterations induced by AD in microglia, nor was the cortical plaque load altered. Visualizing astrocyte morphology in GFAP-eGFP mice showed no clear structural differences in GFAP/VIM null mice, but did show diminished interaction of astrocyte processes with plaques. Microglial proliferation increased similarly in all AD groups. In conclusion, absence of GFAP, or both GFAP and VIM, alters AD-induced changes in gene expression profile of astrocytes, showing a compensation of the decrease of neuronal support genes and a trend for a slightly higher inflammatory expression profile. However, this has no consequences for the development of plaque load, microglial proliferation, or microglial activation. GLIA 2015;63:1036-1056


Keywords

A, astrocytes, GFAP, gliosis, microglia, Vimentin

Last updated on 2019-05-12 at 04:20