Nestin Regulates Neurogenesis in Mice Through Notch Signaling From Astrocytes to Neural Stem Cells.

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Ulrika Wilhelmsson, Isabell Lebkuechner, Renata Leke, Pavel Marasek, Xiaoguang Yang, Daniel Antfolk, Meng Chen, Paria Mohseni, Eva Lasič, Saša Trkov Bobnar, Matjaž Stenovec, Robert Zorec, Andras Nagy, Cecilia Sahlgren, Marcela Pekna, Milos Pekny
Förläggare: Oxford University Press
Publiceringsår: 2019
Tidskrift: Cerebral Cortex
Tidskriftsakronym: Cereb Cortex
ISSN: 1460-2199
eISSN: 1460-2199


Abstrakt

The intermediate filament (nanofilament) protein nestin is a marker of neural stem cells, but its role in neurogenesis, including adult neurogenesis, remains unclear. Here, we investigated the role of nestin in neurogenesis in adult nestin-deficient (Nes-/-) mice. We found that the proliferation of Nes-/- neural stem cells was not altered, but neurogenesis in the hippocampal dentate gyrus of Nes-/- mice was increased. Surprisingly, the proneurogenic effect of nestin deficiency was mediated by its function in the astrocyte niche. Through its role in Notch signaling from astrocytes to neural stem cells, nestin negatively regulates neuronal differentiation and survival; however, its expression in neural stem cells is not required for normal neurogenesis. In behavioral studies, nestin deficiency in mice did not affect associative learning but was associated with impaired long-term memory.


Nyckelord

astrocyte motility, astrocytes, intermediate filaments, mouse, nanofilaments, Nestin, neural stem cells

Senast uppdaterad 2019-19-10 vid 05:02