Fluorescent single-digit detonation nanodiamond for biomedical applications

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Nunn N, d’Amora M, Prabhakar N, Panich AM, Froumin N, Torelli MD, Vlasov I, Reineck P, Gibson B, Rosenholm JM, Giordani S, Shenderova O
Förläggare: IOP Publishing
Publiceringsår: 2018
Tidskrift: Methods and Applications in Fluorescence
Volym: 6
Nummer: 3
Artikelns första sida, sidnummer: 035010
ISSN: 2050-6120


Abstrakt

Detonation nanodiamonds (DNDs) have emerged as promising candidates for a
variety of biomedical applications, thanks to different physicochemical
and biological properties, such as small size and reactive surfaces. In
this study, we propose carbon dot decorated single digit (4–5 nm
diameter) primary particles of detonation nanodiamond as promising
fluorescent probes. Due to their intrinsic fluorescence originating from
tiny (1–2 atomic layer thickness) carbonaceous structures on their
surfaces, they exhibit brightness suitable for in vitro imaging.
Moreover, this material offers a unique, cost effective alternative to
sub-10 nm nanodiamonds containing fluorescent nitrogen-vacancy color
centers, which have not yet been produced at large scale. In this paper,
carbon dot decorated nanodiamonds are characterized by several
analytical techniques. In addition, the efficient cellular uptake and
fluorescence of these particles are observed in vitro on MDA-MD-231 breast cancer cells by means of confocal imaging. Finally, the in vivo
biocompatibility of carbon dot decorated nanodiamonds is demonstrated
in zebrafish during the development. Our results indicate the potential
of single-digit detonation nanodiamonds as biocompatible fluorescent
probes. This unique material will find application in correlative light
and electron microscopy, where small sized NDs can be attached to
antibodies to act as a suitable dual marker for intracellular
correlative microscopy of biomolecules.


Nyckelord

bioimaging, fluorescence microscopy, nanodiamonds

Senast uppdaterad 2019-09-12 vid 02:53