Human dermal fibroblast proliferation controlled by surface roughness of two-component nanostructured latex polymer coatings

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Emil Rosqvist, Erik Niemelä, Arun P. Venu, Ruut Kummala, Petri Ihalainen, Martti Toivakka, John E. Eriksson, Jouko Peltonen
Förläggare: Elsevier
Publiceringsår: 2019
Tidskrift: Colloids and Surfaces B: Biointerfaces
Tidskriftsakronym: ColSurfB
Volym: 174
Artikelns första sida, sidnummer: 136
Artikelns sista sida, sidnummer: 144


Abstrakt

In this study hierarchically-structured latex polymer coatings and self-supporting films were characterised and their suitability for cell growth studies was tested with Human Dermal Fibroblasts (HDF). Latex can be coated or printed on rigid or flexible substrates thus enabling high-throughput fabrication. Here, coverslip glass substrates were coated with blends of two different aqueous latex dispersions: hydrophobic polystyrene (PS) and hydrophilic carboxylated acrylonitrile butadiene styrene (ABS). The nanostructured morphology and topography of the latex films was controlled by varying the mixing ratio of the components in the latex blend. Thin latex-coatings retain high transparency on glass allowing optical and high resolution imaging of cell growth and morphology. Compared to coverslip glass surfaces and commercial well-plates HDF cell growth was enhanced up to 150–250 % on latex surfaces with specific nanostructure. Growth rates were correlated with selected roughness parameters such as effective surface area (Sq), RMS-roughness (Sdr) and correlation length (Scl37). High-resolution confocal microscopy clearly indicated less actin stress-fibre development in cells on the latex surface compared to coverslip glass. The results show that surface nanotopography can, by itself, passively modulate HDF cell proliferation and cytoskeletal architecture.


Senast uppdaterad 2020-03-04 vid 04:05