A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Mehto, Ankelo, Hinkkanen, Mikhailov, Eriksson, Spoof, Meriluoto
Publication year: 2001
Journal: Toxicon
Journal acronym: Toxicon
Volume number: 39
Issue number: 6
Start page: 831
End page: 836
ISSN: 0041-0101
eISSN: 1879-3150


Abstract

An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.

Last updated on 2019-22-09 at 03:50