Cytoskeletal integrity in interphase cells requires protein phosphatase activity

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Eriksson, Brautigan, Vallee, Olmsted, Fujiki, Goldman
Publiceringsår: 1992
Tidskrift: Proceedings of the National Academy of Sciences
Tidskriftsakronym: Proc Natl Acad Sci U S A
Volym: 89
Nummer: 22
Artikelns första sida, sidnummer: 11093
Artikelns sista sida, sidnummer: 11097
ISSN: 0027-8424
eISSN: 1091-6490


Phosphorylation by protein kinases has been established as a key factor in the regulation of cytoskeletal structure. However, little is known about the role of protein phosphatases in cytoskeletal regulation. To assess the possible functions of protein phosphatases in this respect, we studied the effects of the phosphatase inhibitors calyculin A, okadaic acid, and dinophysistoxin 1 (35-methylokadaic acid) on BHK-21 fibroblasts. Within minutes of incubation with these inhibitors, changes are seen in the structural organization of intermediate filaments, followed by a loss of microtubules, as assayed by immunofluorescence. These changes in cytoskeletal structure are accompanied by a rapid and selective increase in vimentin phosphorylation on interphase-specific sites, and they are fully reversible after removal of calyculin A. The results indicate that there is a rapid phosphate turnover on cytoskeletal intermediate filaments and further suggest that protein phosphatases are essential for the maintenance and structural integrity of two major cytoskeletal components.

Senast uppdaterad 2020-30-03 vid 08:55