Lamin and beta-tubulin fragmentation precede chromatin degradation in glutamate-induced neuronal apoptosis

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Ankarcrona, Zhivotovsky, Holmström, Diana, Eriksson, Orrenius, Nicotera
Publiceringsår: 1996
Tidskrift: NeuroReport
Tidskriftsakronym: Neuroreport
Volym: 7
Nummer: 15-17
Artikelns första sida, sidnummer: 2659
Artikelns sista sida, sidnummer: 2664
ISSN: 0959-4965
eISSN: 1473-558X


Abstrakt

During ischaemic brain injury, glutamate accumulation with overstimulation of postsynaptic glutamate receptors and intracellular Ca2+ overload lead to neuronal death. We have shown previously that delayed neuronal death in cultures of cerebellar granule cells (CGCs) exposed to glutamate occurs by apoptosis. Here, we report that lamin cleavage and dissolution of the microtubule network precede chromatin fragmentation in glutamate-induced CGC apoptosis. Like other events that characterize excitotoxic cell death, degradation of lamins, beta-tubulin and disruption of microtubule architecture is inhibited by the NMDA-receptor antagonist MK-801. Our findings suggest that cleavage of key cytoskeletal elements is an important step in glutamate-induced neuronal apoptosis.

Senast uppdaterad 2019-18-08 vid 06:52