Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Sahlgren, Mikhailov, Hellman, Chou, Lendahl, Goldman, Eriksson
Publiceringsår: 2001
Tidskrift: Journal of Biological Chemistry
Tidskriftsakronym: J Biol Chem
Volym: 276
Nummer: 19
Artikelns första sida, sidnummer: 16456
Artikelns sista sida, sidnummer: 16463
ISSN: 0021-9258
eISSN: 1083-351X


The intermediate filament protein nestin is expressed during early stages of development in the central nervous system and in muscle tissues. Nestin expression is associated with morphologically dynamic cells, such as dividing and migrating cells. However, little is known about regulation of nestin during these cellular processes. We have characterized the phosphorylation-based regulation of nestin during different stages of the cell cycle in a neuronal progenitor cell line, ST15A. Confocal microscopy of nestin organization and (32)P in vivo labeling studies show that the mitotic reorganization of nestin is accompanied by elevated phosphorylation of nestin. The phosphorylation-induced alterations in nestin organization during mitosis in ST15A cells are associated with partial disassembly of nestin filaments. Comparative in vitro and in vivo phosphorylation studies identified cdc2 as the primary mitotic kinase and Thr(316) as a cdc2-specific phosphorylation site on nestin. We generated a phosphospecific nestin antibody recognizing the phosphorylated form of this site. By using this antibody we observed that nestin shows constitutive phosphorylation at Thr(316), which is increased during mitosis. This study shows that nestin is reorganized during mitosis and that cdc2-mediated phosphorylation is an important regulator of nestin organization and dynamics during mitosis.

Senast uppdaterad 2020-26-02 vid 03:17