Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Chow, Weis, Kass, Holmström, Eriksson, Orrenius
Publication year: 1995
Journal: FEBS Letters
Journal acronym: FEBS Lett
Volume number: 364
Issue number: 2
Start page: 134
End page: 138
ISSN: 0014-5793
eISSN: 1873-3468


The mechanism of Fas antigen-mediated apoptosis is at present unclear. We show here that the 100,000 x g supernatant from cell lysates prepared from anti-Fas-stimulated JUR-KAT T cells, induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors, TPCK and DCI, and by the interleukin 1-beta-converting enzyme inhibitor, VAD-FMK. In addition, chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK, but not by DCI or VAD-FMK. These results suggest that Fas-mediated apoptosis in T cells involves the activation of a cascade of proteases.

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