Structure and toxicity of a peptide hepatotoxin from the cyanobacterium Oscillatoria agardhii

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Meriluoto, Sandström, Eriksson, Remaud, Craig, Chattopadhyaya
Publiceringsår: 1989
Tidskrift: Toxicon
Tidskriftsakronym: Toxicon
Volym: 27
Nummer: 9
Artikelns första sida, sidnummer: 1021
Artikelns sista sida, sidnummer: 1034
ISSN: 0041-0101
eISSN: 1879-3150


A peptide hepatotoxin was isolated by reversed phase liquid chromatography from the cyanobacterium Oscillatoria agardhii and characterized structurally and toxicologically. Amino acid analyses, proton nuclear magnetic resonance and fast atom bombardment mass spectrometry showed that the toxin is a cyclic heptapeptide (mol. wt 1023.5) with the structure cyclo-(Ala-Arg-Asp-Arg-Adda-Glu-N-methyldehydroAla) (Adda: 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid). In mice the toxic effects were restricted mainly to the liver where the toxin induced massive hemorrhages and a disruption of the lobular and sinusoidal structure. The i.p. LD50 of the toxin was 250 micrograms/kg. The structural and toxic properties of this peptide are very close to those of microcystins, cyclic peptide toxins produced by the cyanobacterium Microcystis aeruginosa.

Senast uppdaterad 2020-23-01 vid 02:44