Quantitative analysis of the self-assembly strategies of intermediate filaments from tetrameric vimentin

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Czeizler E, Mizera A, Czeizler E, Back RJ, Eriksson JE, Petre I
Förläggare: Institute of Electrical and Electronics Engineers
Publiceringsår: 2000
Tidskrift: IEEE/ACM Transactions on Computational Biology and Bioinformatics
Tidskriftsakronym: IEEE/ACM Trans Comput Biol Bioinform
Volym: 9
Nummer: 3
Artikelns första sida, sidnummer: 885
Artikelns sista sida, sidnummer: 898
ISSN: 1557-9964


In vitro assembly of intermediate filaments from tetrameric vimentin consists of a very rapid phase of tetramers laterally associating into unit-length filaments and a slow phase of filament elongation. We focus in this paper on a systematic quantitative investigation of two molecular models for filament assembly, recently proposed in (Kirmse et al. J. Biol. Chem. 282, 52 (2007), 18563-18572), through mathematical modeling, model fitting, and model validation. We analyze the quantitative contribution of each filament elongation strategy: with tetramers, with unit-length filaments, with longer filaments, or combinations thereof. In each case, we discuss the numerical fitting of the model with respect to one set of data, and its separate validation with respect to a second, different set of data. We introduce a high-resolution model for vimentin filament self-assembly, able to capture the detailed dynamics of filaments of arbitrary length. This provides much more predictive power for the model, in comparison to previous models where only the mean length of all filaments in the solution could be analyzed. We show how kinetic observations on low-resolution models can be extrapolated to the high-resolution model and used for lowering its complexity.

Senast uppdaterad 2020-25-02 vid 04:58