Hepatocyte deformation induced by cyanobacterial toxins reflects inhibition of protein phosphatases

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Eriksson JE, Toivola D, Meriluoto JA, Karaki H, Han YG, Hartshorne D
Publiceringsår: 1990
Tidskrift: Biochemical and Biophysical Research Communications
Tidskriftsakronym: Biochem Biophys Res Commun
Volym: 173
Nummer: 3
Artikelns första sida, sidnummer: 1347
Artikelns sista sida, sidnummer: 1353
ISSN: 0006-291X


The cyclic peptide hepatotoxins microcystin-LR, 7-desmethyl-microcystin-RR and nodularin are potent inhibitors of the protein phosphatases type 1 and type 2A. Their potency of inhibition resembles calyculin-A and to a lesser extent okadaic acid. These hepatotoxins increase the overall level of protein phosphorylation in hepatocytes. Evidence is presented to indicate that in hepatocytes the morphological changes and effects on the cytoskeleton are due to phosphatase inhibition. The potency of these compounds in inducing hepatocyte deformation is similar to their potency in inhibiting phosphatase activity. These results suggest that the hepatotoxicity of these peptides is related to inhibition of phosphatases, and further indicate the importance of the protein phosphorylation in maintenance of structural and homeostatic integrity in these cells.

Senast uppdaterad 2020-31-05 vid 03:37