Neuronal cells show regulatory differences in the hsp70 gene response

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: Kaarniranta K, Oksala N, Karjalainen HM, Suuronen T, Sistonen L, Helminen HJ, Salminen A, Lammi MJ
Publiceringsår: 2002
Tidskrift: Molecular Brain Research
Tidskriftsakronym: MOL BRAIN RES
Volym: 101
Nummer: 1-2
Artikelns första sida, sidnummer: 136
Artikelns sista sida, sidnummer: 140
Antal sidor: 5
ISSN: 0169-328X
eISSN: 1872-6941


The synthesis of heat shock proteins (Hsps), encoded by heat shock genes, is increased in response to various stress stimuli. Hsps function as molecular chaperones, they dissociate cytotoxic stress-induced protein aggregates within cells and ensure improved survival. Induction of heat shock genes is mainly regulated at the transcriptional level. The stress responsive transcription factor, heat shock factor 1 (HSF1), is involved in the transcriptional induction of the heat shock genes. Our objective was to examine how hsp70 genes are regulated in different transformed and primary neurons upon exposure to elevated temperature. Our findings reveal that the Hsp70 response is regulated at the translational level in Neuro-2a neuroblastoma cells. while the IMR-32 neuroblastoma cells respond to stress by the classical HSF1-driven transcriptional regulatory mechanism. Primary rat hippocampal neurons show a lack of HSF1 and induction of the hsp70 gene. These observations suggest that neuronal cells display different hsp70 gene expression patterns which range from undetected response to transcriptional and posttranscriptional regulation during heat stress.


heat stress

Senast uppdaterad 2020-01-04 vid 07:32