Pinosylvin and monomethylpinosylvin, constituents of an extract from the knot of Pinus sylvestris, reduce inflammatory gene expression and inflammatory responses in vivo

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Mirka Laavola, Riina Nieminen, Tiina Leppänen, Christer Eckerman, Bjarne Holmbom, Eeva Moilanen
Publisher: AMER CHEMICAL SOC
Publication year: 2015
Journal: Journal of Agricultural and Food Chemistry
Journal acronym: J AGR FOOD CHEM
Volume number: 63
Issue number: 13
Start page: 3445
End page: 3453
Number of pages: 9
ISSN: 0021-8561
eISSN: 1520-5118


Abstract

Scots pine (Pinus sylvestris) is known to be rich in phenolic compounds, which may have anti-inflammatory properties. The present study investigated the anti-inflammatory effects of a knot extract from P. sylvestris and two stilbenes, pinosylvin and monomethylpinosylvin, isolated from the extract. Inflammation is characterized by increased release of pro-inflammatory and regulatory mediators including nitric oxide (NO) produced by the inducible nitric oxide synthase (iNOS) pathway. The knot extract (EC50 values of 3 and 3 mu mu g/mL) as well as two of its constituents, pinosylvin (EC50 values of 13 and 15 mu M) and monomethylpinosylvin (EC50 values of 8 and 12 mu M), reduced NO production and iNOS expression in activated macrophages. They also inhibited the production of inflammatory cytokines IL-6 and MCP-1. More importantly, pinosylvin and monomethylpinosylvin exerted a clear anti-inflammatory effect (80% inhibition at the dose of 100 mg/kg) in the standard in vivo model, carrageenan-induced paw inflammation in the mouse, with the effect being comparable to that of a known iNOS inhibitor L-NIL. The results reveal that the Scots pine stilbenes pinosylvin and monomethylpinosylvin are potential anti-inflammatory compounds.


Keywords

anti-inflammatory, HO-1, iNOS, Pinus sylvestris, stilbene

Last updated on 2019-22-08 at 06:29

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