Studies Related to Norway Spruce Galactoglucomannans: Chemical Synthesis, Conformation Analysis, NMR Spectroscopic Characterization, and Molecular Recognition of Model Compounds

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Filip S. Ekholm, Ana Ardá, Patrik Eklund, Sabine André, Hans-Joachim Gabius, Jesús Jiménez-Barbero, and Reko Leino
Förläggare: WILEY-V C H VERLAG GMBH
Publiceringsår: 2012
Tidskrift: Chemistry - A European Journal
Tidskriftsakronym: CHEM-EUR J
Volym: 18
Nummer: 45
Artikelns första sida, sidnummer: 14392
Artikelns sista sida, sidnummer: 14405
Antal sidor: 14
ISSN: 0947-6539


Abstrakt

Galactoglucomannan (GGM) is a polysaccharide mainly consisting of mannose, glucose, and galactose. GGM is the most abundant hemicellulose in the Norway spruce (Picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. Although several applications for GGM polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported previously. Herein, the synthesis of one of the core trisaccharide units of GGM together with a less-abundant tetrasaccharide fragment is described. In addition, detailed NMR spectroscopic characterization of the model compounds, comparison of the spectral data with natural GGM, investigation of the acetyl-group migration phenomena that takes place in the polysaccharide by using small model compounds, and a binding study between the tetrasaccharide model fragment and a galactose-binding protein (the toxin viscumin) are reported.


Nyckelord

conformation analysis, galactoglucomannan, molecular recognition, NMR spectroscopy, oligosaccharide synthesis

Senast uppdaterad 2020-22-01 vid 04:15