Image Quality Ranking Method for Microscopy

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)


Interna författare/redaktörer


Publikationens författare: Sami Koho, Elnaz Fazeli, John E. Eriksson, Pekka E. Hänninen
Förläggare: NATURE PUBLISHING GROUP
Publiceringsår: 2016
Tidskrift: Scientific Reports
Tidskriftsakronym: SCI REP-UK
Volym: 6
Antal sidor: 14
ISSN: 2045-2322


Abstrakt

Automated analysis of microscope images is necessitated by the increased need for high-resolution follow up of events in time. Manually finding the right images to be analyzed, or eliminated from data analysis are common day-to-day problems in microscopy research today, and the constantly growing size of image datasets does not help the matter. We propose a simple method and a software tool for sorting images within a dataset, according to their relative quality. We demonstrate the applicability of our method in finding good quality images in a STED microscope sample preparation optimization image dataset. The results are validated by comparisons to subjective opinion scores, as well as five state-of-the-art blind image quality assessment methods. We also show how our method can be applied to eliminate useless out-of-focus images in a High-Content-Screening experiment. We further evaluate the ability of our image quality ranking method to detect out-of-focus images, by extensive simulations, and by comparing its performance against previously published, well-established microscopy autofocus metrics.

Senast uppdaterad 2020-24-02 vid 04:01