Weight gain and inflammation regulate aromatase expression in male adipose tissue, as evidenced by reporter gene activity

A1 Originalartikel i en vetenskaplig tidskrift (referentgranskad)

Interna författare/redaktörer

Publikationens författare: L. Polari, E. Yatkin, M.G. Martínez Chacón, M. Ahotupa, A. Smeds, L. Strauss, F. Zhang, M. Poutanen, N. Saarinen, S.I. Mäkelä
Publiceringsår: 2015
Tidskrift: Molecular and Cellular Endocrinology
Tidskriftsakronym: MOL CELL ENDOCRINOL
Volym: 412
Artikelns första sida, sidnummer: 123
Artikelns sista sida, sidnummer: 130
Antal sidor: 8
ISSN: 0303-7207
eISSN: 1872-8057


Obesity and white adipose tissue (WAT) inflammation are associated with enhanced aromatization in women, but little is known about the regulation of aromatase (CYP19A1) gene expression in male WAT. We investigated the impact of weight gain and WAT inflammation on the regulation of CYP19A1 in males, by utilizing the hARO-Luc aromatase reporter mouse model containing a >100-kb 5'-region of the human CYP19A1 gene. We show that hARO-Luc reporter activity is enhanced in WAT of mice with increased adiposity and inflammation. Dexamethasone and TNF alpha, as well as forskolin and phorbol 12-myristate 13-acetate, upregulate hARO-Luc activity, suggesting the involvement of promoters 1.4 and 1.3/II. Furthermore, we show that diet enriched with antioxidative plant polyphenols attenuates WAT inflammation and hARO-Luc activity in obese males. In conclusion, our data suggest that obesity-associated WAT inflammation leads to increased peripheral CYP19A1 expression in males, and that polyphenol-enriched diet may have the potential to attenuate excessive aromatization in WAT of obese men.


Aromatase, Inflammation, Obesity, Polyphenols, White adipose tissue

Senast uppdaterad 2020-24-02 vid 04:32