Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Jiu YM, Lehtimaki J, Tojkander S, Cheng F, Jaalinoja H, Liu XN, Varjosalo M, Eriksson JE, Lappalainen P
Publisher: CELL PRESS
Publication year: 2015
Journal: Cell Reports
Journal acronym: CELL REP
Volume number: 11
Issue number: 10
Start page: 1511
End page: 1518
Number of pages: 8
ISSN: 2211-1247
eISSN: 2211-1247


Abstract

The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal crosslinker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.

Last updated on 2019-13-11 at 04:42