Investigation of protein binding with all solid-state ion-selective electrodes

A1 Journal article (refereed)


Internal Authors/Editors


Publication Details

List of Authors: Alok Prabhu, Johan Bobacka, Ari Ivaska, Kalle Levon
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2013
Journal: Electroanalysis
Journal acronym: ELECTROANAL
Volume number: 25
Issue number: 8
Start page: 1887
End page: 1894
Number of pages: 8
ISSN: 1040-0397
eISSN: 1521-4109


Abstract

A potentiometric sensor for studying charge based adsorption of proteins was created using a single-piece polyaniline-PVC ion-selective electrode (ISE). Three different ISEs, two for Na+ and one for Cl- ion determination, were studied. The Na+-ISEs consisted of a neutral calixarene-based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl- ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA(+)Cl(-)), was used. The Na+ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.


Keywords

Conducting polymers, Electrochemical biosensors, Ion-selective electrodes, Potentiometry, Protein adsorption

Last updated on 2020-02-04 at 07:04