Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

A3 Bokavsnitt, kapitel i forskningsböcker


Interna författare/redaktörer


Publikationens författare: Camilo Guzmán, Maja Šolman, Daniel Abankwa
Redaktörer: Lorenza Trabalzini, Saverio Francesco Retta
Publiceringsår: 2014
Förläggare: Humana Press
Moderpublikationens namn: Ras Signalling
Volym: 1120
Artikelns första sida, sidnummer: 307
Artikelns sista sida, sidnummer: 326
ISBN: 978-1-62703-790-7
eISBN: 978-1-62703-791-4
ISSN: 1064-3745


Abstrakt

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.

Senast uppdaterad 2019-09-12 vid 03:01