Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

A3 Book section, Chapters in research books


Internal Authors/Editors


Publication Details

List of Authors: Camilo Guzmán, Maja Šolman, Daniel Abankwa
Editors: Lorenza Trabalzini, Saverio Francesco Retta
Publication year: 2014
Publisher: Humana Press
Book title: Ras Signalling
Volume number: 1120
Start page: 307
End page: 326
ISBN: 978-1-62703-790-7
eISBN: 978-1-62703-791-4
ISSN: 1064-3745


Abstract

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.

Last updated on 2019-21-08 at 06:37