Ion release, hydroxyapatite conversion, and cytotoxicity of boron-containing bioactive glass scaffolds

A1 Journal article (refereed)

Internal Authors/Editors

Publication Details

List of Authors: Preethi Balasubramanian, Alina Grünewald, Rainer Detsch, Leena Hupa, Bojan Jokic, Francesca Tallia, Anu K. Solanki, Julian R. Jones, Aldo R. Boccaccini
Publication year: 2016
Journal: International Journal of Applied Glass Science
Volume number: 7
Issue number: 2
Start page: 206
End page: 215
eISSN: 2041-1294


We report the development and characterization of boron-releasing highly porous three-dimensional bioactive glass (BG) scaffolds fabricated by the foam replica technique. Three types of bioactive glasses with (wt%) 0.2%, 12.5%, 25% B2O3, and related varying SiO2 contents (wt%): 50%, 37.5%, and 25%, were investigated. The well-known 13-93 (silicate) and 13-93B3 (borate) (in wt% – 56.6% B2O3, 5.5% Na2O, 11.1% K2O, 4.6% MgO, 18.5% CaO, 3.7% P2O5) BGs were used as controls to study the influence of the presence of boron on the mechanical properties, surface reactivity, and cytotoxicity of scaffolds. Surface morphology and surface properties of the BG scaffolds were measured. X-ray diffraction (XRD) analyses showed that the scaffolds of all five compositions were amorphous. The scaffolds with 12.5 wt% B2O3 exhibited satisfactory compressive strength in the range of 1–2 MPa. A dissolution study in cell culture medium was carried out, and ion release profiles, and apatite formation of the scaffolds were assessed. The cytotoxicity of the scaffolds was evaluated using a stromal cell line (ST2). Cells were found to attach and spread well on the scaffolds' surfaces. We conclude that borosilicate scaffolds containing 12.5 wt% B2O3 provide the best combination of properties, including relatively high mechanical strength, apatite formation, and cytocompatibility, and thus, they are promising candidates for bone tissue engineering.

Last updated on 2020-30-05 at 04:43